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Prokaryotic Expreesion and Polyclonal Antibodies Preparation of cdk7 and cyclin H in Zebrafish
Lin Chu1,2, Qing-Yun Liu1, Min Qian2, Yuan-Chang Yan1,3, Yi-Ping Li1*
1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,Shanghai 200031, China; 2School of Life Science, East China Normal University, Shanghai 200062, China;3Model Organi
Abstract: Expression vectors of CDK7 and cyclin H gene in zebrafish were constructed and transformed into E. coli BL21 separately for IPTG induced expression. The results showed that both fusion protein were expressed in the form of inclusion body. Fusion proteins were isolated and then lyophilized to raise the polyclonal antisera against CDK7 and cyclin H respectively by immunizing New Zealand rabbits. The antisera were purified and characterized by ELISA， Western blotting and immunofluorescence-staining. The results showed that the antibodies had high titer， affinity and specificity.
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