High Throughput Sequencing Technologies for DNA Double-Strand Break
WU Shuheng, LIANG Yu, WU Wei*
The DSB (DNA double-strand break) is the most severe type of DNA damage. Studying the underlying mechanisms of DSB generation and repair will help to understand disease initiation and progression. In the past decade, the advent of next-generation sequencing has greatly promoted the development of sequencing-based methods for DSB detection, making it possible to identify the “hotspots” of DSB and measure the repair and processing events. These assays have been widely applied to investigate the roles of many key repair factors, DSB hotspots in different biological contexts, impact of chromatin context on DSB repair, opening a fascinating portal into DSB research. Combined with the laboratory’s research focus, this article will briefly summarize the sources of DSB, their repair pathways, and published genome-wide detection assays for DSB.
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