Identification and Expression Pattern Analysis of BBM Family Genes of Phyllostachys edulis
XU Ruotong, ZHOU Mingbing*
BBM (BABY BOOM) transcription factors have important roles in inducing plant somatic embryogenesis, promoting cell proliferation and regeneration, and improving the efficiency of genetic transformation, and are often applied to improve the efficiency of plant genetic transformation. With the employment of the bioinformatics, this study identified the BBM family members of moso bamboo, and analyzed the phylogenetic relationships. The promoter regulatory elements, physicochemical properties of encoded proteins, gene structures, amino acid conserved sequences of proteins, positions in chromosomes, and their protein secondary and tertiary structures were analyzed; the expression pattern of moso bamboo BBM family members in different tissues and callus regeneration process was analyzed by real-time quantitative RT-PCR. A total of six BBM family genes were identified in Phyllostachys edulis, which were distributed on six different chromosomes. The BBM protein of moso bamboo encodes 486-699 amino acids, and its isoelectric point ranges from 6.31 (PeBBM2) to 8.92 (PeBBM3). Secondary structure prediction of BBM proteins showed that irregular coiled and α-helices accounted for up to about 80%. The phylogenetic tree showed that the PeBBMs were all clustered in the AP2 family, indicating that the BBMs of moso bamboo belonged to the AP2 family. The results of subcellular localization prediction showed that most of moso bamboo BBM proteins were located in the nucleus and the rest in the cytoplasm. Cis-acting element analysis indicated that the transcriptional expression of moso bamboo BBM might be associated with abiotic stress, light response and transcriptional regulation. Real-time quantitative RT-PCR results showed that there was tissue variability in the expression of the PeBBM gene, indicating that different members of the gene family played different roles in the growth and development of moso bamboo, with PeBBMs having the highest relative expression in callus regeneration process. It was hypothesized that PeBBMs might be involved in the process of moso banboo regeneration process. The results of this study laid a certain foundation for the in-depth study of the function of BBM family genes in moso bamboo.
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