Comprehensive Experimental Teaching Practice on Various Exosome Isolation Methods
LI Yanwei*, GUO Chun, ZHOU Nan, HUANG Qiong, WANG Jiajia, CHEN Jingyao, HUANG Yingying, SHEN Xin, YU Xiaomin, SHEN Hongying
FCM (flow cytometry) has become increasingly diverse and versatile with the rapid development of monoclonal antibody technology and optoelectronic technology. However, the isolation and purification of exosomes and other extracellular vesicles have been impeded by the limitations of the detection capabilities of FCM. This study establishes and investigates methods for optimizing exosome sample preparation to enhance the theoretical and practical aspects of FCM teaching, rendering it more intuitive, comprehensible, and feasible. The methods include optimizing FCM configuration and experimental parameters, different exosome isolation methods [ultracentrifugation, FACS (flow cytometric sorting), nanoFCM analysis, and electron microscopy imaging identification]. Through practical application of these methods, students can fully master the relevant detection techniques and data analysis methods, providing support for the application of FCM in exosome isolation and subsequent functional studies. The integration of teaching, research, and practical experience greatly stimulates students’ interest and enthusiasm for learning experimental skills, significantly enhancing their experimental skills and research innovation capabilities, contributing to the cultivation of versatile research and innovation talents in the new era.
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