Effects of Nicardipine on Proliferation, Apoptosis and Chemoresistance of Bladder Cancer Cells by Regulating RhoA/ROCK Signaling Pathway

PANG Yun^1
*, YANG Jingke², LI Rongfang³, YANG Haojie⁴, LI Lili⁵, BAI Tingting³

( ¹ Department of Pharmacy, Xi’an Jiaotong University Hospital, Xi’an 710049, China; ²Department of Urology, Xi’an No.9 Hospital, Xi’an 710054, China; ³ Cardiovascular hospital Department Three, Xi’an No.9 Hospital; ⁴ Public Health Center, Xi’an Jiaotong University Hospital, Xi’an 710049, China; ⁵Physical Examination Center, Xi’an Jiaotong University Hospital, Xi’an 710049, China)

Abstract:

The aim of this study was to investigate the effect of nicardipine on the proliferation, apoptosis and chemoresistance of bladder cancer cells by regulating RhoA/ROCK signaling pathway. T24 cells of bladder cancer were separated into control group, L-nicardipine group, M-nicardipine group, H-nicardipine group, H-nicardipine+LPA group, DMSO group, cisplatin group, H-nicardipine+cisplatin group, H-nicardipine+cisplatin group, and H-nicardipine+cisplatin+LPA group. The proliferation activity, apoptosis, migration, invasion ability, and RhoA/ROCK signaling pathway protein expression of T24 cells were detected using CCK-8 assay kit, flow cytometry, scratch assay, Transwell method, and Western blot, respectively. Compared with the control group, the D value, scratch healing rate, number of invasive cells, and the RhoA and ROCK protein expression levels in the L-nicardipine group, M-nicardipine group, and H-nicardipine group were obviously reduced (P<0.05), while the apoptosis rate of cells was obviously increased (P<0.05). Compared with the H-nicardipine group, the D value, scratch healing rate, number of invasive cells, and the RhoA and ROCK expression levels in H-nicardipine+LPA group were obviously increased (P<0.05), while the apoptosis rate of cells was obviously decreased (P<0.05). Compared with the DMSO group, the D value, scratch healing rate, and number of invasive cells in the cisplatin group were decreased, while the apoptosis rate was increased (P<0.05). Compared with the cisplatin group, the D value, scratch healing rate, and number of invasive cells in the H-nicardipine group and the H-nicardipine+cisplatin group were decreased, while the apoptosis rate was increased (P<0.05). Compared with the H-nicardipine+cisplatin group, the H-nicardipine+cisplatin+LPA group showed an increase in D value, scratch healing rate, and number of invasive cells, and a decrease in apoptosis rate (P<0.05). Nicardipine can inhibit the proliferation and chemoresistance of bladder cancer cells, and promote cell apoptosis. The mechanism may be related to the inhibition of RhoA/ROCK signaling pathway.

CSTR: 32200.14.cjcb.2024.06.0008