Impacts of lncRNA CBR3-AS1 on Proliferation, Apoptosis,and Invasion of Nasopharyngeal Carcinoma Cells by Regulating the miR-145-5p/FSCN1 Axis
WANG Huaxin1, LOU Dan2, YANG Lei2, ZHANG Haizhong3, LIU Lisha1*
This study aims to investigate the impacts of lncRNA CBR3-AS1 (long non-coding RNA CBR3-AS1) on the proliferation, apoptosis and invasion of NPC (nasopharyngeal carcinoma) cells by targeting the miR145-5p (microRNA-145-5p)/FSCN1 (fascin 1) axis. The qRT-PCR method was applied to analyze the expressionlevels of lncRNA CBR3-AS1 and miR-145-5p in nasopharyngeal carcinoma tissues. Nasopharyngeal cancercell CNE-1 was divided into si-NC group, si-CBR3-AS1 group, si-CBR3-AS1+anti-miR-NC group, si-CBR3-AS1+anti-miR-145-5p group, miR-NC group, miR-145-5p mimics group, miR-145-5p mimics+pcDNA group, andmiR-145-5p mimics+FSCN1 group. Double luciferase experiment was applied to detect the targeting relationshipbetween lncRNA CBR3-AS1 and miR-145-5p, and between FSCN1 and miR-145-5p; MTT method was appliedto detect cell proliferation; Annexin V-FITC/PI method was applied to detect cell apoptosis; Transwell experimentwas applied to detect cell invasion ability; Western blot was applied to detect the protein expression changes of P21(cell cycle negative regulator), Bcl-2 (B cell lymphoblastoma-2), Bax (Bcl-2 associated X protein), MMP-2 (matrix metalloproteinase 2), and MMP-9 (matrix metalloproteinase 9). Mouse transplantation tumor experiment wasapplied to explore the effect of LncRNA CBR3-AS1 on the growth of nasopharyngeal carcinoma transplantationtumors and miR-145-5p/FSCN1. In nasopharyngeal carcinoma tissue, the expression of lncRNA CBR3-AS1 wasup-regulated, while the expression of miR-145-5p was down-regulated (P<0.05). Interference with lncRNA CBR3-AS1 or up-regulation of miR-145-5p expression was able to inhibit the proliferation and invasion of nasopharyngealcarcinoma cells, and promote the apoptosis of nasopharyngeal carcinoma cells (P<0.05). Down-regulation of miR145-5p expression or up-regulation of FSCN1 expression was able to reverse the inhibitory effects of interferingwith lncRNA CBR3-AS1 expression or up-regulation of miR-145-5p expression on the proliferation and invasionof nasopharyngeal carcinoma cells (P<0.05). The mouse transplantation tumor experiment showed that down-regulating the expression of lncRNA CBR3-AS1 was able to up-regulate the expression of miR-145-5p, thereby downregulating the expression of FSCN1 and inhibiting the growth of nasopharyngeal carcinoma transplantation tumors(P<0.05). lncRNA CBR3-AS1 is up-regulated in nasopharyngeal carcinoma tissue, down-regulating the expressionof lncRNA CBR3-AS1 can inhibit the proliferation and invasion of nasopharyngeal carcinoma cells, and promotecell apoptosis by regulating the miR-145-5p/FSCN1 axis.
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