Effect of Lidocaine Regulating RhoA/ROCK Axison Biological Behavior of Colorectal Cancer Cells
ZHAO Xin1*, YIN Jian2, JIA Tong3
This study focused on the effects of Lido (lidocaine) on the biological behavior of CRC(colorectal cancer) cells by regulating the RhoA (Ras homologous gene family member A)/ROCK (Rho associated coiled-coil forming protein kinase) axis. In this study, human colorectal cancer cells LS513 were treated with0~1 250 μmol/L lidocaine, and cell viability was detected by CCK-8 method to screen suitable drug concentration.The cells were grouped into Control group, lidocaine low concentration group (Lido-L group, 500 μmol/L Lido),lidocaine medium concentration group (Lido-M group, 750 μmol/L Lido), lidocaine high concentration group(Lido-H group, 1 000 μmol/L Lido) and lidocaine high concentration+ROCK signal pathway activator LPA group (LidoH+LPA group, 1 000 μmol/L Lido+10 μmol/L LPA group). Edu was applied to detect cell proliferation; scratch healing test and transwell chamber test were applied to detect cell migration and invasion ability, respectively; flow cytometry was applied to detect cell apoptosis; Western blot was applied to detect the expression of PCNA, Bax, Bcl2, RhoA, ROCK 1, E-cadherin, and N-cadherin proteins. In this study, compared with 0 μmol/L Lido, the activity ofLS513 cells treated with 500 μmol/L, 750 μmol/L, 1 000 μmol/L and 1 250 μmol/L Lido obviously decreased (P<0.05),lidocaine of 500 μmol/L, 750 μmol/L and 1 000 μmol/L was selected for subsequent experiments. Compared withthe Control group, the Edu positive rate, scratch healing rate, cell invasion number, and PCNA, N-cadherin, Bcl2, RhoA, and ROCK 1 protein expression of LS513 cells in the Lido-L group, Lido-M group, and Lido-H groupdecreased sequentially (P<0.05), the apoptosis rate, E-cadherin and Bax protein expression increased sequentially(P<0.05); compared with the Lido-H group, the Edu positive rate, scratch healing rate, cell invasion number, and PCNA,N-cadherin, Bcl-2, RhoA, and ROCK 1 protein expression of LS513 cells in the Lido-H+LPA group obviously increased(P<0.05), the apoptosis rate, E-cadherin, and Bax protein expression were obviously reduced (P<0.05). Lidocaine mayinhibit the malignant biological behavior of colorectal cancer cells by inhibiting RhoA/ROCK signaling pathway.
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