Mechanism of miR-106b-5p on Invasion and Migration of Oral Squamous Cell Carcinoma through Targeted Regulation of SIRT7/SMAD4 Signaling Pathway

This study aimed to investigate the effects and mechanism of miR-106b-5p on migration and invasion of OSCC (oral squamous cell carcinoma) cells. The expression of miR-106b-5p in OSCC tissues and cell lines was detected by qRT-PCR. SCC15 and OECM1 cells were divided into Control group, miR-NC group, miR-106b-5p mimics group, anti-miR-NC group, anti-miR-106b-5p group, anti-miR-106b-5p+si-NC group, and anti-miR-106b-5p+si-SIRT7 group, respectively. Cell proliferation, migration and invasion were detected in each group; Western blot was used to detect the protein expression of E-cadherin, N-cadherin, MMP-9, SIRT7 and SMAD4; double luciferase reporter gene assay and RIP assay verified the targeting relationship between miR-106b-5p and SIRT7. The results showed that the expression levels of miR-106b-5p in OSCC tissues and cells were increased (P<0.05). Overexpression of miR-106b-5p could significantly promote the proliferation, migration, invasion and EMT of OSCC cells, and inhibition of miR-106b-5p expression could significantly inhibit the proliferation, migration, invasion and EMT of OSCC cells (P<0.05). Double luciferase reporter gene experiment and RIP experiment confirmed that there was a targeting relationship between miR-106b-5p and SIRT7 (P<0.05). Inhibition of SIRT7 expression could reverse the inhibitory effects of miR-106b-5p expression on the proliferation, migration, invasion and EMT of OSCC cells (P<0.05). In conclusion, the expression of miR-106b-5p is up-regulated in OSCC tissues and cells. Inhibition of miR-106b-5p expression can inhibit the proliferation, migration, invasion and EMT of OSCC cells by regulating SIRT7/SMAD4 signal pathway

CSTR: 32200.14.cjcb.2024.02.0007