Application of Mass Spectrometry-Based Proteomics in Organelle Research
WANG Hongli1,2, SHI Dongxue1,2, CHEN Yang1,2*
Organelles are specialized compartments within eukaryotic cells with distinct structures and biological functions. In-depth analysis of protein abundance, protein spatial distributions, interactions, and transportations in different organelles is crucial for understanding organelle functions and disease mechanisms. Mass spectrometry-based proteomics has been extensively utilized for identification and quantification of various complexed biological samples, and hence, is widely applied in organelle research. Mass spectrometry-based proteomics accompanied by centrifugation or proximity labeling, has played a central role in advancing our understanding of organelle proteomes, inter-organelle transportations, post-translational modifications, and the mechanisms underlying various diseases. The demand for proteomic technologies in organelle research is growing, and the proteomics of trace samples will potentially have a tremendous application in mapping disease-related organelle proteome and deciphering the functions of organelles in diseases in the future.
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