Microencapsulated Newborn Rats Ovaries in Vitro and Allotransplantation

Abstract: To explore the feasibility of alginate-polylysine-alginate (APA) microencapsulated newborn rats ovaries in treatment of castrated adult female rats. The APA microencapsulated newborn rats ovaries were prepared with a high-voltage electrostatic system. The microcapsules were cultured in vitro， and the estradiol (E₂) and progesterone (P) secretion were measured periodically， and the ovaries were measured by transmission electron microscope periodically. The microcapsules were transplanted to castrated adult female rats， and the E₂ and P secretion were measured periodically. The recovery and maintaining time of the oestrus cycle were carefully observed， and the endocrine function of transplanted ovaries was determined by measuring serum E₂ and P. The transplanted microcapsules were recovered at intervals. The microcapsules prepared in the same condition of high-voltage electrostatic system were round and homogeneous. The E₂， P secreted by microencapsulated newborn rats ovaries were shown the permeate of the membrane of APA microcapsules， and the develop of granulose cells in ovaries were founded by transmission electron microscope in vitro. After microcapsules were transplanted， the level of E₂， P in castrated adult female rats were raised gradually， but the oestrus cycle were not resumed. The majority of recovered APA microcapsules were intact， and no evidence of immunological reaction in terms of fibrosis. The E₂ and P were secreted continually and steadily by the APA microencapsulated newborn rats ovaries prepared by high-voltage electrostatic system. The ovarian function of castrated adult female rats were improved by APA microcapsules， and the microcapsules have good biocompatibility in vivo.
    

CSTR: 32200.14.cjcb.2006.05.0025