The Improvement of Cell Biology “Cell Agglutination Reaction” ExperimentTeaching and Agglutination Judgment Method
ZHOU Zhihua, LU Guo, HE Yongjin, ZHAO Dongyue, CHEN Hanbing, LI Qin*
In this study, the conventional undergraduate cell biology experiment “cell agglutination reaction” experiment was comprehensively expanded, and sugar inhibition experiment was incorporated into the “cellagglutination reaction” experiment to help students fully understand and master the characteristics of lectin and theglycobase components on the cell surface. By improving the experimental method, 0.4% Trypan blue was used tostain the cells in the experiment and then observed with an inverted microscope, the initial concentration of cell agglutination could be accurately determined. the method of judging the degree of agglutination was optimized, andthe proportion of cell junction and cell dispersion was used to determine the degree of cell agglutination, which could analyze the experimental results more accurately. By improving the teaching method, the hot social issuesand ideological and political elements can be organically introduced into the classroom, and the students’ interest inindependent learning can be fully enhanced. Through the improvement and exploration of the above four aspects,students can more directly observe the adhesion phenomenon between cells under the action of lectins, and further understand the specific binding of lectins to sugar sites and the types and functions of sugar groups on thecell plasma membrane on the basis of reasonable and accurate analysis of the experimental results. Cultivate students’ ability to discover and solve problems independently, and improve the experimental teaching effect. 0.4%Trypan Blue staining microscopy method and cell agglutination degree judgment method provide a more convenient method for undergraduate experimental teaching and research workers to visually observe the phenomenonand difference of cell agglutination in the future. and also provide a basis and innovative direction for accuratelyjudging the definition of initial concentration of cell agglutination under low concentration of lectin, and alsoprovide a basis and innovative direction for accurately judging the definition of initial concentration of cell agglutination under low concentration of lectin.
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