In Vitro Study of Tumor Cell Membrane Camouflaged NanomedicineMediated Ovarian Cancer Therapy with High PD-1 Expression
LI Wei1,2, GAO Xiaohan3, SUN Zhihong2, LIU Jie2, SUN Chengming1,2,3*
In this study, a novel biomimetic nanomedicine (PDT@DNPs) was prepared and its killing effect on ID8 cell of ovarian cancer and its reversing effect on exhausted T cells were evaluated. The membrane oftumor cells with high expression of PD-1 was modified on the surface of DSPE-PEG-MAL and DDP (cisplatin)self-assembled particle skeleton to construct a biomimetic nano-delivery vector PDT@DNPs. The morphology,particle size, potential and other properties were characterized and determined by transmission electron microscopy,nanoparticle particle size and potential analyzer. ID8 cells were selected as the study objects. Flow cytometry, laserconfocal imaging, CCK-8 (cell counting kit-8) cytotoxicity test, Calcein-AM and PI (propyl iodide) staining wereused to evaluate the targeting and killing ability of PDT@DNPs. In addition, the release level of LDH (lactate dehydrogenase) and cytokine secretion levels in the co-incubation system of T cells and ID8 cells were detected, andthe regulatory ability of PDT@DNPs on the anti-tumor immunity of T cells was analyzed. These results indicatedthat the novel bionic nanomedicine PDT@DNPs constructed in this study had good ID8 cell targeting and killingability, and could enhance the tumor cell killing ability of T cells in the co-incubation system.
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