Research Progress in Protein Complex Expression and Purification Technology
LI Siwei1,2, LI Jia2, ZANG Yi1,3*
Protein complex, the functional molecule of cell, appears to play a central role in signal transduction and functional regulation of organism. The structure of protein complex is of great significance for studying the molecular mechanism of protein-protein interaction, predicting protein function, discovering potential drug targets and contributing to drug design. Three methods are mainly used for purifying protein complexes so far. One major technique is to obtain complex components separately and assemble in vitro; another is co-expression of protein components in the same host cell following by assembling in vivo; the third one is purification of endogenous protein complexes, notably through the use of knock-in method. Here, this study summarizes three major techniques for isolating protein complex and anticipates to provide reference for further researches on important protein complex structure.
CN
EN