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The Effect of Physiological Mechanical Strain upon Osteoprotegerin/Receptor Activator of NF-κB Ligand mRNA Expression by Murine Stromal Cells
Li Liu*, Xiao-Cong Zhang, Xi-Cong Zhen, Lie-Feng Zhang
The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China
Abstract: The recent identification of the receptor activator of NF-κB ligand (RANKL)， its cognate receptor RANK， and its decoy receptor osteoprotegerin (OPG) has led to a new molecular perspective on osteoclasts biology and bone homeostasis. To investigate the effect of physiological mechanical strain upon OPG/RANKL mRNA expression by murine stromal cells and further elucidate the mechanism of mechanical effect on osteoclasts， the OPG/RANKL genes expression was analyzed by semi-quantitative reverse-transcript polymerase chain reaction (RT-PCR) after time-controlled mechanical strain applied to murine stromal cells. The results showed that mechanical strain enhanced the expression of OPG genes to 73% after 9 h applying， while reduced the expression of RANKL genes to 34.4% after 6 h applying respectively. It is suggested that strain-induced OPG/RANKL genes expression tendency by murine stromal cells may contribute to explain the mechanism of osteolysis prevention by physiological mechanical strain.
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