Exploration on Innovative Mode and Practice of Cell Biology Experiments—Taking the Design of a Comprehensive Experiment on Labelling and Observation of the Cytoskeleton as an Example
YAN Zhen, YANG Jun, PENG Rui, LI Chenghua, YI Peishan, NIU Mengmeng*
The exploration and practice of student-centered hierarchical experimental teaching of cell biology experiments is conducive to improving the comprehensive quality of students, and plays a positive role in the reform of experimental teaching method in colleges and universities. The distribution and localization of cytoskeleton is an important part of the cell biology experiments, but the comprehensive experimental teaching design of cytoskeleton labelling and observation has rarely been reported. In this study, a comprehensive experiment on cytoskeleton labelling and observation were designed using cultured animal cells as materials, the contents of which covered the distribution of microtubules and microfilaments in cells, the immunolabelling technology of cellular microtubules, fluorescence probe labelling of microfilament, and the factors affecting the morphology and distribution of the cell cytoskeleton. The experiment project consist of four parts: two basic experiments to observe the distribution of microtubules and microfilaments of human cervical cancer cells using immunofluorescence method or phalloidin labelling method respectively, one expansion experiment to explore the factors affecting the cytoskeleton distribution, and one innovative experiment to observe the cytoskeleton of rat mesenchymal cells (C6 cells), all of which constitute the hierarchical teaching of the comprehensive experiment in cell biology experiment course. Teaching practice of this comprehensive experiment shows that the student-centered hierarchical teaching stimulates students’ interest in learning, improves their motivation and initiative in learning, enhances their ability to analyze and solve problems and apply knowledge comprehensively, playing critical roles in cultivating students’ innovative and practical ability and comprehensive ability.
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