Fish Embryonic Stem Cell Culture and Related Biotechnology
XU Jia, LI Shifeng, LI Yiping*
ESCs (embryonic stem cells) have self-renewal capacity and multidirectional differentiation potential. It provides an ideal experimental material for generating targeted mutant transgenic animals, studying biological processes and revealing embryonic development and regenerative medicine. This sparked intense interest in exploring ESCs in different species. ESC lines have been established in fish, rodents and primates. This review summarizes the technologies and applicational difficulties of ESCs and ESCs-like cell lines in fish, discusses following issues including long-term culture in vitro compromise pluripotency of ESCs-like cell lines isolated from fish embryos, low efficiency of germline transmission due to abnormal chromosome karyotype and asynchronous reprogramming. Nuclear transplantation and semi-clonal techniques are proposed as alternative routes to germline chimerism and rapid preparation of transgenic strains of fish models, in the hope of providing a reference for optimizing fish ESCs culture
CN
EN