In Vitro Tumor Stem Cell Enrichment Modality

LI Xuexue¹, WEI Xudong^1,2*, CHENG Xiaoling¹

( ¹ The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China; ²Department of Otorhinolaryngology, Head and Neck Surgery, Gansu Provincial People’s Hospital, Lanzhou 730000, China)

Abstract:

Cancer remains the second leading cause of death in the world, despite the increasing availability of therapies. Studies have shown that the difficulty in cancer treatment is mainly due to the high heterogeneity of tumors and drug resistance. The development of drugs targeting CSCs (cancer stem cells) can reduce the mortality rate of cancer patients, and thus targeting CSCs has become a new type of anticancer therapy. However, in the study of stem cells and cell-cell interactions, stem cells are still difficult to be captured, insufficiently captured, and unable to grow in accordance with the expected growth trend after capture. Therefore, the accurate simulation of in vitro experiments into in vivo experiments is still a bottleneck in the study of stem cells and tumors. In this paper, the main methods and recent progress of stem cell enrichment are summarized, in order to provide a feasible reference for the future research of CSCs.

CSTR: 32200.14.cjcb.2023.08.0015