Optimization and Regulation of Adenine Base Editors
ZHANG Chi1,2#, HE Ziyan1,2#, YANG Yudong1,2#, SONG Yixuan1,2, ZHANG Mingliang1,2*
ABE (adenine base editor) is a single-base editing system based on the CRISPR/Cas system. ABEs are constructed by fusing artificially evolved TadA* (adenine deaminase) with Streptococcus pyogenes Cas nickase to achieve A·T>G·C single-base substitutions in genomic DNA. Since its development, ABE has undergone multiple rounds of evolution to improve its editing efficiency. Meanwhile, a series of regulatory factors targeting structural modules of ABE or related cellular pathways have been identified, enabling spatial-temporal control of their activity and greatly expanding their applications. Currently, ABEs, as well as regulatory factors, have shown great potential in basic research and clinical applications. This article reviews the evolution of ABE and the investigation of their regulatory approaches, and prospects for the development of adenine base editing technology
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