Comparison, Analysis and Application of the Existing Lysosomal Isolation and Purification Methods
BI Jundong1,2, ZHENG Lingling1,2, YANG Mei1,2*, WANG Yonghua1,2*
Lysosomes, as important organelles in eukaryotic cells, are not only the site of degradation of endogenous and exogenous substances, but also the center of cellular energy sensing and regulation, and are capable of coordinating the transport, metabolism and secretion of cellular substances. An imbalance in lysosomal homeostasis can lead to many diseases, such as lysosomal storage disorders, tumors, immunodeficiency and neurodegenerative diseases. Obtaining complete and highly pure lysosomes is an important prerequisite for the study of their microstructure, homeostatic regulation and related molecular functions. Currently, commonly used lysosomal isolation and purification techniques include centrifugal isolation and purification, fluorescence-assisted organelle sorting, affinity immunopurification, magnetic nanoparticle isolation and purification, and Lyso-IP. This article reviews the principles, characteristics and applications of the existing lysosomal purification techniques, and makes a comparative analysis of them.
CN
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