Influence of miR-425-5p on Cisplatin Sensitivity of Ovarian Cancer Cells by Regulating the PTEN/PI3K/AKT Axis
SONG Yufang, ZHANG Qian, LIU Yingjie, LIU Fang*
This article explored the influence of miR-425-5p (microRNA-425-5p) on DDP (cisplatin) sensitivity of OC (ovarian cancer) cells by regulating PTEN (phosphatase and tensin homolog)/PI3K (phosphatidylinositol-3-kinase)/AKT (protein kinase B) axis. Human OC resistant cells A2780/DDP were randomly divided into DDP group, DDP+miR-425-5p inhibitor group, DDP+NC (negative control) inhibitor group, DDP+miR-425-5p inhibitor+PIC (vanadium peroxide, PTEN inhibitor) group, A2780 cells were used as Control group. The cell proliferation ability of each group was detected by CCK-8 method. The cell migration ability of each group was detected by cell scratch assay. The invasive ability of cells in each group was detected by Transwell assay. The apoptosis rate of each group was analyzed by flow cytometry. The expression of miR-425-5p, as well as PTEN, PI3K and AKT mRNA in each group of cells was determined by real-time quantitative PCR. The expression of PTEN/PI3K/AKT axis-related proteins in each group of cells was determined by Western blot. The dual-luciferase gene reporter assay was used to detect the targeting relationship between miR-425-5p and PTEN. The results showed that, compared with Control group, the cell proliferation ability (48 h, 72 h), migration rate and cell invasion number in DDP group were significantly increased (P<0.05), and the apoptosis rate was significantly decreased (P<0.05). Compared with the DDP group, the cell proliferation ability (48 h, 72 h), migration rate, cell invasion number, miR-425-5p expression level, PI3K and AKT mRNA expression and protein phosphorylation levels in the DDP+miR-425-5p inhibitor group were significantly decreased (P<0.05), and the apoptosis rate, PTEN mRNA and protein expression were significantly increased (P<0.05). PIC significantly attenuated the effect of miR-425-5p knockdown on DDP sensitivity of A2780 cells (P<0.05). There was a targeting relationship between miR-425-5p and PTEN. Down-regulation of miR-425-5p may increase the sensitivity of OC cells to DDP by activating the PTEN/PI3K/AKT pathway.
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