Effects of Microcystin-LR on the Microstructure of Tissues and HO-1,IL-10R1 Gene Expression of Grass Carp (Ctenopharyngodon idella)
QIN Jiangfeng
This project mainly studies the effect of MC-LR (microcystin-LR) on grass carp (Ctenopharyngodon idella). The dose of MC-LR injected intraperitoneally to grass carp was 100 μg/kg , the tissues and blood of grass carp were sampled after 0.5 h, 1 h, 3 h, 6 h, 12 h and 24 h. Then used for the study of tissue structure and the expression of HO-1 and IL-10R1 genes. (1) The results of microscopic structure showed that the intestinal villus epithelial cells were exfoliated, goblet cells were increased, and the nuclei of epithelial cells were deformed; intestinal congestion occurred in the experimental group at 6 h; the lamina propria of intestinal villi mucosa was separated in the 12 h and 24 h experimental groups. MC-LR has a time effect on grass carp. (2) The HO-1 and IL-10R1 genes were detected by fluorescence quantitative PCR. The results showed that the expression of HO-1 genes in the gill, liver, intestine, spleen and heart of grass carp were significantly decreased compared with the control group (P<0.05). The expression of IL-10R1 genes in the grain, liver, intestine, spleen and blood of grass carp were significantly decreased compared with the control group (P<0.05). MC-LR causes pathological damage to the intestine, liver, and gills of grass carp, and inhibits the genes expression of HO-1 and IL-10R1, providing theoretical knowledge of the relevant mechanisms for MC-LR
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