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Construction of Mouse Annexin II Gene Knockout Recombinant and Generation of Mouse Annexin II_/_ Cell Line
Ying-Chun Hou*, Aiko Hirata1, Fusao Hirata1
College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China;1College of Pharmacy Sciences, Wayne State University, Detroit, Michigan 48201, USA
Abstract: Annexins are Ca2+ and phospholipids binding proteins forming an evolutionary conserved multigene family with members of the family being expressed throughout animal and plant kingdoms. Some members of the family participate in the regulation of membrane organization， membrane traffic， and the regulation of ion (Ca2+) currents across membranes or Ca2+ concentration within cells. Recently， several cellular or animal annexin knockout models as well as dominant-negative mutants of the family have been established. As an important member of annexin family， annexin II has been proved to be involved with and play some important roles in the development of some human diseases. The paper reports that a gene knockout recombinant (pPNT/annexinII/LacZ) was constructed for blocking the mouse annexin II expression， and two cell clones (D4 and E2) were screened out as the annexin II-/- L5178Y cell clones that will be confirmed further more using PCR. The annexin II knockout recombinant and the annexin II-/- cell line can be used to generate annexin II null mice and further researches on annexin II in future.
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