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Flow Cytometry Detection and Sorting of Microglia in the Nervous System
JIANG Bowen, CHEN Lingling*
(Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China)
Abstract:
Microglia are the main immune cells in CNS (central nervous system), representing 5%-10% of cells in the brain. As the macrophage, they constantly scavenge the CNS for plaques, damaged or unnecessary neurons and synapses, and infectious agents. Isolation of microglia from CNS tissue provides a powerful tool to study basic cell biology and examine the effects of in vivo treatments on microglia function. This article describes approaches for purification of microglia by FACS (fluorescence-activated cell sorting) using microglia-specific surface markers.
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