Effects of CHST11 Gene on Biological Behavior of Gastric Cancer SGC-7901 Cells
SHI Wen1*, HAN Wei2, SUN Huanhuan1, LIU Mengying1, MA Fuquan1
The effects of CHST11 gene on the biological behavior of gastric cancer SGC-7901 cells were investigated. qRT-PCR and Western blot methods were used to detect CHST11 mRNA and protein expression in gastric cancer cells after the stable silencing of CHST11 gene. The cells were transfected with CHST11-interfering sh-CHST11 plasmid and control NC plasmid, and the CHST11 mRNA expression in gastric cancer cells after CHST11 gene knockout was detected by qRT-PCR. By the MTT assay and the plate cloning experiment, the proliferation and cloning capacities of the transfected gastric cancer cells separately were identified. Flow cytometry was used to identify the apoptosis of gastric cancer cells following transfection. The capacity of stomach cancer cells to migrate and invade was determined using the scratch healing test and the Transwell assay, respectively. The results showed that the expression of CHST11 gene was up-regulated in gastric cancer cells. The capacity of SGC-7901-sh-CHST11 cells to proliferate, migrate and invade after stable transfection were significantly lower than that of SGC-7901 cells without intervention, and the number of apoptosis of SGC-7901-sh-CHST11 cells was significantly increased. The proliferation, migration, invasion and apoptosis of SGC-7901-NC cells were not significantly different from that of the untreated SGC-7901 cells. The results showed that CHST11 gene may enhance gastric cancer cell proliferation, migration, and invasion, as well as prevent apoptosis and promote the incidence and growth of gastric cancer cells.
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