Flow Cytometric Analysis of Regulatory T Cells from Various Mouse Tissues

FCM (flow cytometry) is a cutting-edge methodology used to characterize the cellular and molecular phenotypes of different cell groups in single-cell suspension. It is a powerful tool that is currently widely used in immunological research. With the advantages of high throughput and multi-parameters, FCM plays a vital role in the analysis of immune cell phenotypes. Treg (regulatory T cell), whose specific transcription factor is Foxp3, is a type of T cell subsets that suppresses immune response and maintains immune homeostasis. Many of tissue-specific Foxp3+ Treg groups have unique biological functions in different tissues. This article aims to use FCM to identify and analyze the T cell subpopulations in various tissues of mice, isolate Treg from spleen, liver, lung, colon, lymph nodes and other tissues of mice, and analyze the proportion and proliferation of Treg, so as to provide a reference for in-depth understanding of the function of tissue-specific Treg.

CSTR: 32200.14.cjcb.2023.06.0009