Molecular Mechanism of LINC00963 Targeting miR-1224-5p Regulating Proliferation and Radiosensitivity of Breast Cancer Cells

This study investigates the molecular mechanisms of LINC00963 targeting miR-1224-5p regulating proliferation and radiosensitivity of breast cancer cells. qRT-PCR was used to detect the relative expression of LINC00963 and miR-1224-5p in breast epithelial cells MCF-10A and breast cancer cells (MDA-MB-231, MDAMB-468, MCF-7). Divide MDA-MB-231 cells into si-NC group (transfected with si-NC), si-LINC00963 group (transfected with si-LINC00963), miR-NC group (transfected with miR-NC), miR-1224-5p group (transfected with miR-1224-5p), si-LINC00963+anti-miR-NC group (co-transfected with si-LINC00963 and anti-miR-NC), siLINC00963+anti-miR-1224-5p group (co-transfected with si-LINC00963 and anti-miR-1224-5p). The expression of LINC00963 and miR-1224-5p was detected by qRT-PCR assay. CyclinD1 and PCNA protein levels were assessed by Western blot assay. Cell proliferation was measured using MTT assay. Cell radiosensitivity was detected by cloning assay. The targeting relationship between LINC00963 and miR-1224-5p was confirmed using a dual luciferase reporter experiment. In breast cancer cells, the relative expression of LINC00963 was significantly increased, and the relative expression of miR-1224-5p was significantly reduced. Inhibition of LINC00963 and overexpression of miR-1224-5p reduced breast cancer cell proliferation activity and cell survival score, and down-regulate CyclinD1, PCNA protein expression. miR-1224-5p acted as a target of LINC00963. Interference miR-1224-5p reversed the effects of LINC00963 knockdown on breast cancer cell proliferation and radiosensitivity. LINC00963 might improve breast cancer cell radiosensitivity and suppress cell proliferation by targeting miR-1224-5p.

CSTR: 32200.14.cjcb.2023.06.0008