Effect of CircOGDH on Hypoxia Reoxygenation Induced Neuronal Damage through miR-24-3p Mediated HOXA1 Upregulation

This study aimed to investigate the mechanism of CircOGDH on hypoxic reoxygenation (H/R) induced neuronal damage by targeting and regulating HOXA1 through miR-24-3p. Ht22 cells were used as the research object, hypoxia reoxygenation was used to induce neuronal damage, and the cells were divided into negative control group (si-NC group), CircOGDH silencing group (si-CircOGDH group), overexpression negative control group (miR-NC group), miR-24-3p overexpression group (miR-24-3p minic group), CircOGD silencing+inhibitor negative control group (si-CircOGDH+anti-miR-NC group), and CircOGDH silencing +miR24-3p inhibitor group (si-CircOGDH+anti-miR-24-3p group). Control group and H/R group without transfection were also set up. The expression of CircOGDH, miR-24-3p and HOXA1 mRNA was detected by qRT-PCR; the cell activity was detected by CCK-8 method; the oxidative stress level was detected by micro method; apoptosis was detected by flow cytometry; Western blot was used to detect the expression of HOXA1, Bax and Bcl-2 proteins. The results showed that the expression of CircOGDH and HOXA1 was up-regulated and the expression of miR-24-3p was down-regulated in Ht22 cells in H/R group, the apoptosis rate, the expression of Bax protein, and the contents of LDH and MDA increased, the cell survival rate, the expression of Bcl-2 protein, the activities of SOD and GSH-Px decreased (P<0.05); silencing CircOGDH could up-regulate the expression of miR-24-3p and down-regulate the expression of HOXA1 in H/R-induced Ht22 cells, improve cell survival rate and the levels of Bcl-2 protein, SOD, and GSH-Px, reduce the apoptosis rate, Bax protein, the contents of LDH, MDA (P<0.05); overexpression of miR-24-3p could reduce the expression of HOXA1, increase the cell survival rate, the levels of Bcl-2 protein, SOD, GSH-Px, and reduce the apoptosis rate, Bax protein, contents of LDH and MDA (P<0.05); inhibiting the expression of miR-24-3p could partially reverse the protective effect of silencing CircOGDH on HT22 cells damage. In conclusion, silencing CircOGDH may up-regulate the expression of miR-24-3p, downregulate the expression of HOXA1, improve the oxidative damage of neurons induced by H/R, and inhibit their apoptosis.

CSTR: 32200.14.cjcb.2023.06.0004