Design of Comprehensive Biotechnology Experimental Course Based on the Scientific Literacy Cultivation—Take the Study of the NLRP3 Inflammasome Domain as an Example
SUN Cencen1, LI Xuyun1, YU Xiaoyun1, FANG Yu1, WANG Mengling1, MEI Ruhuan1, GU Yifeng2*
A comprehensive biotechnology experimental course aiming at the cultivation of scientific literacy was designed, which was composed of scientific research lectures, basic experiments and innovative experiments. Scientific research ideas and methods were introduced in lectures, which would help students form a correct, rigorous and complete scientific research framework; the basic experiment takes NLRP3 inflammasome as the research object. Based on the basic logic of scientific research, the structural domain and function of NLRP3 inflammasome are studied using bioinformatics, molecular cloning, cell biology and immunology methods; based on the results and methods of basic experiments, the innovation experiment further explored the function and structure of NLRP3. The project covers multi-disciplinary key experimental technologies. Scientific research lectures, basic experiments and innovative experiments are organically integrated to ensure the cultivation of key experimental technologies and the deepening understanding of relevant theoretical knowledge. At the same time, it provides a large space for innovation, which is conducive to students to establish good thought and habits for scientific research, and provides students with relatively complete scientific research training. At the same time, the project has mature technology, stable results and high feasibility, which is worth promoting.
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