Establishment and Preliminary Study of Comprehensive Experiment of “Different Preparation Methods and Flow Cytometry Detection of Mouse Spleen Single Cell Suspension”
HUANG Yingying*, XING Yueting, Sawuti Ayixibu, YANG Dan, LI Yanwei, WANG Jiajia, GUO Chun, ZHOU Nan
Due to the complexity of sample preparation, instrument manipulation and data analysis involved in FCM (flow cytometry) applications, beneficial explorations on the content and form of FCM experimental teaching were conducted. Accordingly, a comprehensive experiment of “different preparation methods and FCM detection of mouse spleen single cell suspension” has been established. The new method can not only include sampling of mouse primary tissue, different preparation methods of single-cell suspension (manual dissociation, automatic dissociation), flow cytometer counting, microscopic observation, etc., but also cover many knowledge points, such as color matching and labeling of antibodies in FCM, operation of flow cytometer, data analysis (cluster of T lymphocyte subsets), etc. The influences of different dissociation methods on the experimental results were discussed. It greatly improves the effectiveness and quality of experimental teaching, gives full play to the important supporting role of FCM in teaching and scientific research in colleges and universities. Based on these lessons, students can learn the complete technology chain of FCM, which enhances their scientific research ability and creativity. The comprehensive experiment would provide impetus for the cultivation of compound scientific research talents.
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