Impacts of Stachydrine on Proliferation, Migration and Invasion of Endometrial Carcinoma Cells by Regulating CXCL12/CXCR4 Axis
LIU Ying1,2*, CAI Yue1
This study mainly discusses the impacts of STA (stachydrine) on proliferation, invasion and migration of EC (endometrial carcinoma) cells and the regulatory mechanism of STA on CXCL12/CXCR4 axis. EC cells were separated into control group, CXCL12/CXCR4 inhibitor group (AMD 3100 group), low and high concentration STA groups, and high STA+CXCL12 group. CCK-8 was applied to detect cell proliferation; clonogenic assay was applied to detect the ability of cell cloning; the apoptosis was detected by flow cytometry; Transwell assay was applied to detect the migration and invasion of cells; Western blot was applied to detect the expression of E-cadherin, Vimentin, CXCL12, CXCR4 in cells; the EC transplanted tumor model of mice was established and the tumor growth of mice in each group was observed. Results found, compared with the control group, the cell proliferation activity, clone number, migration and invasion numbers, and the expression of Vimentin, CXCL12, CXCR4 proteins in AMD 3100 group and STA treatment group decreased, the apoptosis rate and the expression of E-cadherin protein increased (P<0.05); compared with the STA high concentration group, the cell proliferation activity, clone number, migration and invasion numbers, and the expression of Vimentin, CXCL12, CXCR4 proteins in high STA+CXCL12 group increased, the apoptosis rate and the expression of E-cadherin protein decreased (P<0.05); In vivo experiments, the tumor weight and volume of AMD 3100 group and STA treatment group were lower than those of Control group (P<0.05). Therefore, STA inhibits the proliferation, migration and invasion of EC cells by inhibiting CXCL12/CXCR4 axis.
CN
EN