Effects of Sufentanil on the Proliferation, Migration and Invasion of Colon Cancer SW1116 Cells by Regulating LncRNA PSMA3-AS1

CHEN Yinghao¹, TANG Jianjun², ZHANG Yinhui³, WU Wenchun^4
*

( ¹ Jinzhou Medical University, Jinzhou 121001; ²Department of General Surgery, Xiangyang NO.1 People’s Hospital, Xiangyang 441000, China; ³ Department of Orthopedics, Wanshun Orthopedic Hospital, Xiangyang 441000, China; ⁴ Department of Anesthesiology, Xiangyang Hospital of Traditional Chinese Medicine, Xiangyang 441000, China)

Abstract:

This article aims to explore the effect of sufentanil on the proliferation, migration and invasion of colon cancer SW1116 cells and its possible mechanism. Cultured human colon cancer cells SW1116 in vitro, randomly divided into groups: control group, low sufentanil group, medium sufentanil group, high sufentanil group, si-NC group, si-LncRNA PSMA3-AS1 group, high sufentanil+pcDNA group, high sufentanil+pcDNA-LncRNA PSMA3-AS1 group. The cell proliferation was detected by CCK-8 method and clonal formation experiment; migration and invasion were detected by Transwell. The expression level of LncRNA PSMA3-AS1 was detected by qRT-PCR. The expressions of E-cadherin and N-cadherin proteins were detected by Western blot. After low, medium and high sufentanil treatment or downregulation of LncRNA PSMA3-AS1 expression, the survival rate of colon cancer SW1116 cells, LncRNA PSMA3-AS1 expression level and the level of N-cadherin were decreased (P<0.05), the number of colony formation, migration, and invasion of cells was decreased (P<0.05), and the level of E-cadherin was increased (P<0.05); the up-regulated expression of LncRNA PSMA3-AS1 reduced the effects of cassufentanil on the proliferation, clonogenesis, migration and invasion of colon cancer SW1116 cells. Sufentanil could reduce the proliferation, clone formation, migration and invasion of colon cancer cells by down-regulating the expression of LncRNA PSMA3-AS1.

CSTR: 32200.14.cjcb.2023.04.0009