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Tandem Affinity Purification and Its Application
Qi-Hua Hong, An-Guo Chen*
College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
Abstract: Tandem affinity purification (TAP) is a new method developed recently for the purification of protein complex under native condition. Proteins purified through two successive affinity chromatography steps can be identified directly by mass spectrometry. The TAP strategy was originally developed using yeast protein complexes as a model system and has been successfully applied in many other organisms. This review focuses on the principle of TAP， the TAP tags and its application in various cells or organisms so far.
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