lncRNA DBH-AS1 Affects the Proliferation and Apoptosis of Infantile Hemangioma Endothelial Cells by Targeting miR-1291
QIN Jiajia, GUO Ying, WU Jun, LIU Yang*
This study aims to investigate the effect of lncRNA (long non-coding RNA) DBH-AS1 on the proliferation and apoptosis of infantile HemECs (hemangioma endothelial cells) and its possible mechanism. Hemangioma tissue and normal subcutaneous tissue adjacent to tumor in 49 infants were collected. qRT-PCR was used to detect the expression of DBH-AS1 and miR-1291 in hemangioma tissue and adjacent normal subcutaneous tissue, and Pearson correlation analysis was used to evaluate the correlation between the expression levels of DBH-AS1 and miR-1291 in hemangioma tissues of infant hemangioma patients. Cell proliferation was detected by CCK-8 assay and clonal formation assay. Cell apoptosis was detected by flow cytometry. The expressions of proliferation and apoptosis related proteins were detected by Western blot. Dual-luciferase reporter assay was used to verify the regulatory relationship between DBH-AS1 and miR-1291. The expression of DBH-AS1 in hemangioma tissue was higher than that in adjacent normal subcutaneous tissue (P<0.05), while the expression of miR-1291 was lower than that in adjacent normal subcutaneous tissue (P<0.05). Pearson correlation analysis showed that the expression level of DBH-AS1 in hemangioma tissue was negatively correlated with that of miR-1291 (r=–0.887, P<0.01). Compared with the si-NC group or miR-NC group, the proliferation activity of HemECs and the protein expression levels of Ki-67 and PCNA in the si-DBH-AS1 group and miR-1291 group were reduced (P<0.05), but the apoptosis rate and the protein expression levels of cleaved-caspase9 and cleaved-caspase3 were increased (P<0.05). DBH-AS1 could target miR-1291, and the expression level of miR-1291 in HemECs in the si-DBH-AS1 group was higher than that in the si-NC group (P<0.05). Downregulation of miR-1291 reversed the effect of interfering DBH-AS1 on the proliferation and apoptosis of HemECs (P<0.05). Interference with DBH-AS1 expression inhibits HemECs proliferation and promotes apoptosis, possibly through the negative regulation of miR-1291.
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