PARP15 Expression in Lung Adenocarcinoma and Its Effect on the Proliferation and Apoptosis of Lung Adenocarcinoma Cells

This paper investigated the clinical significance of PARP15 overexpression in lung adenocarcinoma and its effect on lung adenocarcinoma cell growth and apoptosis. The expression levels in lung adenocarcinoma cells and normal cells were compared by using the UALCAN and GEPIA databases, and the effect of PARP15 gene on the prognostic survival of lung adenocarcinoma patients was analyzed by using the GEPIA database. Next, the core plasmid pCDH-PARP15 was constructed. Stable PARP15 overexpression strains were obtained by lentiviral packaging and infection in human lung adenocarcinoma cell lines A549 and H1299, and overexpression was identified by Western blot. CCK-8 and clone formation assay were used to detect the effect of overexpression of PARP15 on the growth of A549 and H1299 cells. The effect of PARP15 on apoptosis and cell cycle of A549 and H1299 cells were detected by flow cytometry. PARP15 gene expression was all reduced in lung adenocarcinoma cells (P<0.05), and PARP15 gene expression was positively correlated with good prognosis of lung adenocarcinoma patients (P=0.003 6). PARP15 overexpression inhibited the proliferation of lung adenocarcinoma cells (P<0.05) and induced apoptosis (P<0.05), but had no significant effect on cell cycle. PARP15 may play a role in inhibiting the growth of lung adenocarcinoma cells by inducing apoptosis.

CSTR: 32200.14.cjcb.2023.04.0001