The Application of APEX/APEX2-Based Proximity Labeling in MP Research

MPs (membrane proteins) are involved in various complex life activities in human body. Understanding the structure, function and interaction network of MPs is the premise of revealing how the proteins participate in complex biological processes. With the continuous development of a series of technologies in protein structure prediction and analysis, the number of MPs whose structures have been cracked has been increasing steadily. However, information about the structure and function of MPs is still very scarce. Recently, the proximity labeling technology based on engineering ascorbate peroxidase (APEX/APEX2) has been widely used to explore the subcellular localization and topology of MPs, track the dynamic migration of MPs, and find the interacting proteins of MPs. This method provides a high-throughput method for the system analysis of space constrained MPs. This review summarizes the application of APEX/APEX2-based proximity labeling in MP research and provides some methods and technical references for the in-depth study of MPs.

CSTR: 32200.14.cjcb.2023.02.0015