Recent Progress on Protein O-Glycosylation in Plants

ZHANG Yueqian^1,2, SU Xiao^1,2, ZHANG Geng^1,2, ZHANG Xueping^1,2, LI Xiaojuan^1,2*

(¹Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Bioscience and Bioengineering, Beijing Forestry University, Beijing 100083, China; ²Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China)

Abstract:

Glycosylation, including N-glycosylation, O-glycosylation, and glycosylphosphatidylinositol anchoring, is one of the most important post-translational modifications of proteins. O-glycosylation occurs widely in plant cells. It is not only involved in regulating protein transcription and signal transduction, but also closely related to cell wall synthesis. Among various kinds of O-glycosylated modifications, O-N-acetylglucosamine (O-GlcNAc) glycosylation which has specific structure is easy to be detected and characterized. Therefore, many techniques have been developed to detect and characterize these proteins. However, the structure and function of other O-glycosylated proteins still remain to be further elucidated. Here, this article reviews the research progresses of different types of O-glycosylated modifications in plants. Moreover, it summarizes the advantages and disadvantages of different technologies. Finally, the challenges and future directions in studying O-glycosylated protein in plant biology are discussed.

CSTR: 32200.14.cjcb.2023.02.0008