Effects of LncRNA MALAT1 Regulating miR-383-5p on Proliferation, Migration and Invasion of Neuroblastoma Cells
XIONG Yingliang, WANG Haixin, PENG Tao, LI Jianhua*
This study is aimed to investigate the effect of lncRNA (long non-coding RNA) MALAT1 (metastasis associated in lung denocarcinoma transcript 1) on the proliferation, migration and invasion of NB (neuroblastoma) cells. NB tissues and adjacent non-cancer tissues of 45 NB patients treated in the First People’s Hospital of Liangshan Yi Autonomous Prefecture from January 2019 to December 2021 were selected. The expression levels of MALAT1 and miR-383-5p in NB tissue and adjacent non-cancer tissue, human umbilical vein endothelial cells HUVECs and NB cell lines (NMB, SHEP21N, and SHEP2) were detected by qRT-PCR. SHEP2 was seletced as the research object, and was randomly grouped into si-NC group, si-MALAT1 group, pc-NC group, pc-MALAT1 group, miR-NC group, miR-383-5p overexpression group (OE-miR-383-5p group), anti-NC group, anti-miR-383-5p group, si-MALAT1+anti-NC group and si-MALAT1+anti-miR-383-5p group. The proliferation of SHEP2 cells in each group was detected by CCK-8 method. The protein expression levels of Cyclin D1, PCNA, MMP-2 and MMP-9 in SHEP2 cells of each group were detected by Western blot assay. The migration and invasion of SHEP2 cells in each group were detected by Transwell assay. Dual luciferase reporter assay verified the targeting relationship between MALAT1 and miR-383-5p. The expression of MALAT1 was up-regulated and miR-383-5p was down-regulated in NB tissues and cell lines (NMB, SHEP21N, SHEP2), with the highest expression of MALAT1 and the lowest expression of miR-383-5p in SHEP2 cells. Knockdown of MALAT1 expression or over-expression of miR-383-5p significantly reduced SHEP2 cell proliferation, the number of migrating cells and the number of invasive cells, and down-regulated the expression of Cyclin D1, PCNA, MMP-2 and MMP-9 proteins. MALAT1 can target and regulate the expression of miR-383-5p, and inhibiting the expression of miR-383-5p can reverse the inhibitory effect of knocking down MALAT1 on the proliferation, migration and invasion of SHEP2 cells. The knockdown of MALAT1 can inhibit the proliferation, migration and invasion of SHEP2 cells, and its mechanism of action may be related to the targeted regulation of miR-383-5p.
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