Influences of lncRNA CASC11 on Proliferation, Apoptosis, Migration and Invasion of Gastric Cancer Cells by Targeting miR-498

This paper discusses the effect of lncRNA (long non-coding RNA) CASC11 (cancer susceptibility candidate 11) on the proliferation, apoptosis, migration and invasion of GC (gastric cancer) cells and the molecular mechanism. Normal human gastric mucosal cell lines (GES-1) and human GC cell lines (MKN-45, KATOIII, MKN7 and HGC-27) were cultured in vitro. The expressions of CASC11, miR-498 (microRNA-498) and LIN28B mRNA in the cells were detected by RT-qPCR (real-time quantitative reverse transcription polymerase chain reaction). MKN7 cells were divided into control group (NC group), si-NC group, si-CASC11 group, si-CASC11+antiNC group, and si-CASC11+anti-miR-498 group. si-CASC11, si-NC, miR-498 inhibitor, and inhibitor-NC were transfected into cells using Lipofectamine 3000 transfection reagent. After transfection, RT-qPCR were used to detect the expressions of CASC11, miR-498 and LIN28B mRNA in cells. Western blot was used to detect the protein expressions of LIN28B in cells. Cell proliferation activity was detected by CCK-8 assay. Apoptosis was detected by flow cytometry. Transwell assay was used to detect cell migration and invasion abilities. Dual-luciferase and RIP (RNA immunoprecipitation) experiments were used to verify the relationship between CASC11 and miR-498. The effect of CASC11 silence on the growth of MKN7 cells in vivo was detected in BALB/c nude mouse transplantation experiment. The results show that, CASC11 and LIN28B were overexpressed in GC cells, and miR-498 was underexpressed in GC cells (P<0.05). Knockdown of CASC11 could significantly up-regulate miR-498, inhibiting the mRNA and protein expression of LIN28B, inhibiting the proliferation, migration and invasion of GC cells, and inducing GC cell apoptosis (P<0.05); in vivo experiments confirmed that knockdown of CASC11 could inhibit the growth of transplanted tumors in nude mice (P<0.05). Down-regulating the expression of miR-498 could reduce the inhibitory effect of CASC11 silence on proliferation, migration and invasion of GC cells and the growth of transplanted tumors in vivo by increasing the expression of LIN28B (P<0.05). Dual-luciferase and RIP experiments confirmed that miR-498 was a potential target of CASC11. The study concluded that, CASC11 might promote LIN28B expression by acting as a sponge for miR-498, thereby affecting the proliferation, apoptosis, migration and invasion of GC cells.

CSTR: 32200.14.cjcb.2023.02.0004