The Expression of LINC00342/miR-505-3p/PGK1 Gene Expression in Breast Cancer Patients with Tumor Metastasis and Its Influence on Cell Biological Characteristics
GUO Yingye*
Long non-coding RNA LINC00342 has been shown to be involved in important biological functions in a variety of cancers. However, the role and mechanism of LINC00342 in breast cancer remain unclear. This study selected 28 paired tumor tissue and adjacent normal tissue from breast cancer patients, as well as breast cancer cells and normal breast epithelial cells. The expressions of LINC00342, miR-505-3p, PGK1 (phosphoglycerate kinase 1) mRNA were detected by qRT-PCR. The protein expression of PGK1 was detecetd by Western blot. The breast cancer MCF-7 cells were divided into NC group (blank), si-LINC00342 group (transfected with siLINC00342), si-NC group (transfected with si-NC), miR-505-3p group (transfected with miR-505-3p mimic), miR-NC group (transfected with miR-NC), si-PGK1 group (transfected with si-PGK1), si-NC group (transfected with si-NC), si-LINC00342+pcDNA-PGK1 group (simultaneous transfection with si-LINC00342 and pcDNA-PGK1), si-LINC00342+pcDNA group (simultaneous transfection with si-LINC00342 and pcDNA). Western blot was used to detect the expression of PGK1, MMP2 (matrix metalloprotease 2), and MMP9 protein. The MTT method was implemented to monitor the cell proliferation ability, and the Transwell method was employed to assess the number of cell migration and invasion. The dual luciferase activity assay detects the targeted binding between miR-505-3p, LINC00342 and PGK1. The results showed that LINC00342, PGK1 mRNA, and PGK1 protein were significantly up-regulated in breast cancer tissues and cells, while miR-505-3p was significantly down-regulated. LINC00342 interference, miR-505-3p overexpression or PGK1 knockdown could inhibit the proliferation, migration, invasion, and the expression of MMP2 and MMP9 proteins in breast cancer cells. In addition, LINC00342 can directly target miR-505-3p to regulate the expression of PGK1, and high expression of PGK1 can reverse the inhibitory effect of low expression of LINC00342 on the proliferation, migration, and invasion of MCF-7. This study suggests that interference with the expression of LINC00342 may regulate the expression of PGK1 by targeting miR-505-3p to inhibit the proliferation, migration, and invasion of breast cancer cells.
CN
EN