Effect of Aconitine on Calcium Signal in Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes
YAN Meihui, YU Tingting, LI Sen*
This study aims to investigate the effect of aconitine on calcium signal in hiPSCs-CMs (human induced pluripotent stem cells-derived cardiomyocytes). CCK-8 assay was used to determine the effect of aconitine on the survival rate of human cardiomyocytes. The effect of aconitine on the beating frequency of cardiomyocytes under different dosage time and concentration conditions was recorded by confocal microscope light field mode. The effect of aconitine on calcium signal was recorded by confocal microscope fluorescence system under spontaneous and 1, 2, 3 Hz electrical stimulation conditions. To investigate the effect of aconitine on calcium pool, caffeine was used to induce the release of calcium ions from the sarcoplasmic reticulum of cardiomyocytes. Aconitine had toxic effects when the addition dosage was 9 μmol/L and the dosage time was 6 h, which significantly affected the survival rate of human cardiomyocytes. Aconitine could significantly accelerate the beat frequency of hiPSCsCMs, which was related to the drug treatment time and concentration. Aconitine could significantly reduce the amplitude of spontaneous calcium transients and calcium transients under 1, 2, 3 Hz electrical stimulation in human cardiomyocytes. Sarcoplasmic reticulum calcium content of human cardiomyocytes treated with 0.3 μmol/L aconitine for 3 h was decreased. Aconitine had elution effect on the pulsatile frequency and calcium signal of hiPSCsCMs. Aconitine can accelerate the beat frequency of hiPSCs-CMs, reduce the amplitude of calcium transient and the sarcoplasmic reticulum calcium content, and thus affect the myocardial function.
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