Strategies and Regulatory Mechanisms for Maintaining the Stemness of Mesenchymal Stem Cells

JING Hua^1,2, LIN Kaili², ZHAO Cancan²*, ZHAO Bin¹*

(¹School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China; ²Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China)

Abstract:

MSCs (mesenchymal stem cells) are pluripotent stem cells with the potential to maintain selfrenewal and multi-directional differentiation, and have the characteristics of low immunogenicity and high portability. They are an important source of ideal seed cells for tissue engineering. A large number of seed cells are needed in tissue engineering, so it is very important to amplify MSCs in vitro. The in vitro culture of MSCs is mainly based on traditional 2D (two-dimensional) culture. However, in the process of traditional two-dimensional culture, MSCs often occur self-differentiation, which causes them to lose stemness. The stemness of MSCs can be maintained by 3D (three-dimensional) culture methods such as bioreactor, ultra-low adsorption surface, hanging drop culture or scaffold culture. This paper mainly starts from the 3D culture method, and describes its stemness maintenance effect, advantages and related mechanisms on MSC

CSTR: 32200.14.cjcb.2022.11.0009