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Expression Level of Transgene cry1Ab in Different Organs of Sorghum
Mei-Zhen Lu, Hai-Rui Cui*, Yan-Jia Shao, Yang Zhou, Yan-Ling Yao, Ji-Qiang Jin
Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China
Abstract: Expression level of cry1Ab gene in different organs of transgenic sorghum was detected by using Cry1Ab/Cry1Ac QuantiPlate Kits. There were obvious differences in Cry1Ab protein content in the different organs， with the highest in the leaf sheath， followed by the leaf， the glume， the seed， the root and the stem in order， while no Cry1Ab protein was detected in the anther. There were also obvious differences in Cry1Ab protein content in the leaf， the leaf sheath and the stem fragment at different positions. The Cry1Ab protein content in middle leaves was higher than that in top and base leaves， and higher Cry1Ab protein content was found in the middle leaf sheathes followed by the base and top leaf sheathes. However， the highest in the stem fragment was detected in the base.
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