The Application of Flow Cytometry in Analysis of Cancer-Associated Fibroblast Proliferation

FU Rong¹, SHI Zhaopeng², MI Jun^2
*

( ¹Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University College of Basic Medicine Sciences, Shanghai 200025, China; ²Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University College of Basic Medicine Sciences, Shanghai 200025, China)

Abstract:

CAF (cancer-associated fibroblast) are the most numerous stromal cells in tumor tissues and play an essential role in the occurrence and development of tumors. This paper used FCM (flow cytometry) to compare and analyze the differences in cell proliferation and DNA ploidy between cancer-associated fibroblasts and NAF (non-activated fibroblasts). Based on cell morphology observation and cell count, quantitative detection was performed by FCM after staining with PI, EdU and Ki-67, the obtained data were statistically analyzed by GraphPad software. The study showed that compared with NAF cells, the proliferation of CAF cells was slowed down; the cell cycle was blocked in the G0/G1 phase; and the difference was statistically significant (P<0.05). In this paper, FCM is used for the first time to quantify the biological characteristics of CAF cells. It tries to provide a theoretical basis for further mechanism research.

CSTR: 32200.14.cjcb.2022.09.0009