Effective Generation of the Fluorescent Mouse Model Responding to IFNβ Signal using the Sleeping Beauty Transposon System
CAI Shaoli, XU Shan, JIAO Changfeng, FENG Zhihua, LIANG Wangwang, DENG Xiangling, CHEN Qi*, SHEN Yangkun*
IFNβ (interferon β) has an indispensable role in antiviral, immune regulation and oncological therapy. In recent years, numerous evidences have suggested that IFNβ can improve the capacity of the antigen presentation of DC (dendritic cell), as well as significantly increase the activity of T cells. However, it is not clear how IFNβ induces the systematic immune responses during viral infection and tumorigenesis. For this reason, it is vital to find out what types of cells release IFNβ and detect the expression level of IFNβ in various organs. Here, the “Sleeping beauty” transposon/transposase system is applied to construct a genetically modified mouse that is able to express mCherry driven by the IFNβ promoter. The exogenous sequences remain unchanged and expressed following several generation in the mice as verified by PCR and flow cytometry. These data indicate that the mouse model enabling stable expression of mCherry controlled by the IFNβ promoter is successfully constructed. This mouse model provides a useful tool for studying the regulation and function of IFNβ signaling pathway related to its antiviral and antitumor activities
CN
EN