RP11-495P10.1 Affects the Proliferation of HCC Cells by Regulating the Expression of APIP
QI Qi1, GUO Linchi2, YU Xiaoguang1 *
This study investigated the effect of RP11-495P10.1 on the proliferation of HCC cells by regulating the expression of APIP. Firstly, the expression of RP11-495P10.1 in HCC cells was down-regulated by RNAi technology, cells were detected by CCK-8 and colony formation assay, downstream target genes were screened by RNA-seq assay, and they were identified by qRT-PCR and Western blot. Secondly, the mRNA expression of APIP was detected by bioinformatics prediction and liver cancer tissue microarray. RNAi technology was used to interfere with the target gene APIP in HCC cells. Cell proliferation was detected by CCK-8 and colony formation assay. Meanwhile, the expression of proliferation-related genes protein PCNA and CyclinD1 was detected by Western blot. Finally, cell proliferation was detected after RP11-495P10.1 interfered with simultaneous APIP overexpressed. The results showed that the proliferation ability of cells was reduced after interfering with the expression of RP11- 495P10.1, and APIP was the downstream target gene of RP11-495P10.1. In addition, APIP mRNA was highly expressed in HCC tissues, and the mRNA and protein expressions of APIP were decreased after interfering with RP11-495P10.1. What’s more, cell proliferation and the expression of PCNA and CyclinD1 also decreased after the interference of APIP expression. Further overexpression of APIP could reverse the inhibitory effect of RP11- 495P10.1 on the proliferation of HCC cells. In conclusion, this study revealed that RP11-495P10.1 could affect the proliferation of HCC cells by regulating the expression of the target gene APIP, providing a new target for the diagnosis and treatment of HCC.
CN
EN