The Antimicrobial Peptide Aoattacin can Promote the Wound Healing on Burn Wound Mice

LIU Liu¹, LIU Hui¹, WANG Lijun², HE Lifang³, LI Xiaofei¹, CAO Jianping⁴, ZHENG Minghui^1,4*

( 1 Zunyi Medical University, Zunyi 563003, China; 2 Medicine & Technology School of Zunyi Medical University, Zunyi 563003, China; 3 Qiannan Medical College for Nationalities, Guizhou 558000, China; 4 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China)

Abstract:

To study and test the therapeutic effect of antimicrobial peptide Aoattacin on scald infection of model mice, it was expressed and purified with Bac-to-Bac system expression system in insect cell sf9, and the II degree burns infection model to detect the healing of burn wounds mouse were established. Experiment group was treated with antimicrobial peptide once a day (25 mg/L), and the negative control group was treated with PBS (30 mg/L), while Silver Sulfadiazine (10 mg/L) is positive control group. Wound healing rate was calculated by measuring the area of skin lesions after burns (days 7, 14, 21). Wound and surrounding skin were taken for HE staining, and the wound pathological changes were observed to analyze the pathological tissue structure of wound, and expression change in EGF and bFGF was measured by immunohistochemistry methods. The results showed that antimicrobial peptide Aoattacin was successfully expressed and purified with important antibacterial activities. Seven days after burning injury, compared to control group, the colony count (CFU) of the wound surface in antimicrobial peptide mouse had a remarkable reduce (P<0.01) and cruing rate had a significant increase in the antimicrobial peptide group (P<0.05). Compared with the control group, the number of capillaries and fibroblasts in the wound tissue of the antibacterial peptide group increased. Organizational layering is obvious at 14 and 21 days. The expression of EGF and bFGF in the antimicrobial peptide group was significantly higher than that in the control group at each time point (P<0.05). The results showed that the antimicrobial peptide Aoattacin could inhibit burn wound infection and promote wound healing in mice, which thereby promoted the repair process of wounds, and indicated that Aoattacin had the potential to be developed into a drug for treating burns.

CSTR: 32200.14.cjcb.2022.09.0001