Misidentification and Cross-Contamination of Cell Lines in Culture and Their Detection Methods
ZHANG Tongtong, LIAN Ruitong, ZHU Yujie, ZHOU Yanzhu, YUE Jing, ZHUANG Anqi, WU Yihang*
The application of misidentified and cross-contaminated cell lines has caused by very serious consequence in scientific study and clinical application etc. It has plagued us for decades at home and abroad. Cell cross-contamination is the contamination of cell lines with unrelated cells from another cell lines (including the same species or other species) during cell isolation, cultivation and application. Detection for misidentification and cross-contamination of cell lines is the critical control points to ensure the quality of target cells with related products in culture. It is also one of the items that must be examined in approval process of clinical research related to cell therapy and cell-based therapeutic products. However, no reliable detection and evaluation system for mislabeled and cross-contaminated cell lines is available so far. Therefore, this paper reviewed the current status on detection methods for misidentification and cross-contamination of cell lines in the following six aspects such as morphological features, karyotyping, isoenzyme analysis, human leukocyte antigen typing, DNA-based authentication and cell-specific characteristics. Furthermore, to provide important references for the stablishment of quality control system of cell lines used for scientific research and clinical therapy as well as related therapeutic products, the paper also analyzed the existing problems in verification and contamination detection of cell lines.
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