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RNAi of par-3 Gene in C. elegans Deliverd by Feeding Bacteria
Yu-Rong Yang*, Ya-Jun Gao
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Life Science School,iamen University, Xiamen 361005, China
Abstract: The hairpin dsRNA expression vector targeting par-3 gene was constructed and transformed into E.coli HT115. The development of C.elegans embryo was observed after feeded bacteria which contain par-3 dsRNA plasmids for 24 h in 25 °C. The results showed that this construct of RNAi targeting par-3 gene delivered by feeding this bacteria is efficient. The bacteria mediated RNAi of par-3 result in the loss of the asymmetry of the C. elegans early embryo and the change of the spindle orientation in the second cleavage. This construct can be applied into the further study of gene expression in transgenetic worms via bacteria mediated RNAi.
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