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Apoptosis and Expression of Apoptosis Genes during Mouse Early Embryonic Development
Wei Li, Lian-Qing Wang, Jing-Yan Su, Li-Li Yuan, Yun-Qin Yan*
Animal Histology and Embryology Unit, Northeast Agriculture University, Harbin 150030, China
Abstract: There are a lot of apoptosis in early embryo of mouse， and it is induced by expression of apoptotic genes. In our experiment， we used Comet assay to observe the apoptosis during mouse early embryonic development， and nested RT-PCR and Immunocytochemistry to observe the expression of Bcl-2 family members (Bax， Bcl-2， Bak， Bcl-xl). The result shows that the ratio of apoptosis gradually increase， while the cell number increase. In the whole process， Bax doesn't change much， the expression of Bcl-2 increase， while the expression of Bak， Bcl-xl decrease. The research on the expression of apoptotic genes during mouse early embryonic development has great significance to discover the mechanism of embryonic development.
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