Toxic Effects of Genetically Engineered Recombinant Human Metallothionein-III on Cells and Caenorhabditis elegans
SUN Zuoyi1, YUAN Hailiang2, QIN Jianxin2, LI Qiang3, XUE Yuying1*
This study aimed to investigate the toxic effects of different concentrations of rh-MT-III (recombinant human metallothionein III) on HaCaT (human keratinocytes) and L929 cells as well as on C. elegans (Caenorhabdities elegans). The functional data of safety and toxicity, including cell viabilities, cell morphology, cell membrane damages, apoptosis levels of HaCaT and L929 cells, and nematode survival, head swinging and body bending frequency, body length and width, pharyngeal pumping frequency and defecation interval of C. elegans were observed, respectively. For the cell experiments, cells were exposed to rh-MT-III solution at concentrations ranging from 0 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL for 24 h. For the nematode experiments, 0 μg/mL, 5 μg/mL, 50 μg/mL, 500 μg/mL rh-MT-III were used to treat L1 phase nematodes for 72 h. The results showed that there was no significant difference of the functional data between the rh-MT-III treatment and the control groups. These results indicated that rh-MT-Ⅲ at doses of 0~200 μg/mL and 0~500 μg/mL had no obvious toxicity to both two cells and C. elegans, respectively.
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